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Free Serial Dilution Calculator — Volumes & Diagram

Keep concentrations and transfer volumes consistent from the preparation table to the dilution diagram.

Serial dilution calculator

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Serial dilution calculator

Calculate the concentration, transfer and diluent volumes for an equal-volume serial dilution.

Intermediate tubes retain the prepared volume minus the onward transfer. The last tube retains its full prepared volume. Concentration units stay unchanged.

Displayed values use up to eight significant digits. CSV retains the calculated precision.

TubeConcentrationCumulative factorTransfer volumeDiluent volumeRemaining volume
1100 µg/mL10100 µL900 µL900 µL
210 µg/mL100100 µL900 µL900 µL
31 µg/mL1000100 µL900 µL900 µL
40.1 µg/mL10000100 µL900 µL1000 µL

Free Serial Dilution Calculator — Volumes & Diagram— templates & examples

Updated

Free scientific tools

Free Serial Dilution Calculator — Volumes & Diagram

Keep concentrations and transfer volumes consistent from the preparation table to the dilution diagram.

A serial dilution calculator applies a repeated dilution factor to a starting concentration. SciFig calculates each tube’s concentration, cumulative factor, transfer volume, diluent volume and remaining volume, with a corresponding tube diagram. Enter the concentration, factor, step count and freshly mixed volume in consistent units. Review the result table and tube figure, then download the calculation as CSV, export the diagram or save the inputs as JSON.

Serial dilution series comparing 1000 and 2000 microliter prepared volumes
  • Calculate every concentration in the series
  • Plan transfer and diluent volumes
  • See what remains after the next transfer
  • Export the calculation and tube diagram

Concentrations, Transfers and Remaining Volume

Separate the per-step factor, cumulative dilution and remaining tube volume in a shared calculation.

Calculate every concentration in the series

Enter the starting concentration, per-step dilution factor and number of steps. Each concentration is divided by the factor again at the next step. The table also reports the cumulative factor, making the difference between one dilution step and the entire series visible. The serial dilution diagram displays each tube alongside its calculated concentration and volume.

  • Per-tube concentration
  • Cumulative dilution factor
  • One to 24 steps
Enter the series conditions

Plan transfer and diluent volumes

Set the volume immediately after mixing each tube. The transfer is that volume divided by the dilution factor; fresh diluent makes up the difference. Every calculation keeps the selected concentration unit and one volume unit, so a display change does not imply a mass-to-molar conversion.

  • Transfer volume per step
  • Fresh diluent volume
  • Consistent chosen units
Set the prepared volume

See what remains after the next transfer

The table distinguishes freshly prepared volume from the volume remaining after an aliquot leaves for the next tube. Intermediate tubes retain less than their freshly mixed volume. The final tube retains its full prepared volume because the plan does not include an onward transfer from that tube.

  • Intermediate-tube remaining volume
  • Final-tube volume
  • Explicit preparation convention
Review remaining volumes

Export the calculation and tube diagram

SVG and PNG show the dilution sequence with concentrations and transfer labels. CSV retains the calculated table values; JSON saves the inputs and units for a reproducible plan. The diagram and downloads use the same valid calculation as the table.

  • SVG and PNG tube diagram
  • CSV calculation table
  • Restorable input JSON
Go to export controls

How to Plan the Dilution Series

Enter concentration, factor, steps and mixed volume, then review the table and export the plan.

  1. Set the starting concentration

    Enter the concentration and keep the appropriate unit for the series. Select a finite dilution factor greater than one and between one and 24 steps.

  2. Set the mixed volume

    Enter the volume immediately after mixing each tube and choose µL or mL. The transfer and fresh diluent amounts are calculated in that same volume unit.

  3. Review concentration and remaining volume

    Read the per-tube and cumulative dilution results. Distinguish the freshly mixed volume from the amount left after transferring an aliquot to the next tube.

  4. Download the calculation

    Export the table as CSV or the tube figure as SVG or PNG. Save project JSON to restore the inputs and units for a subsequent calculation.

Dilution Plans for Laboratory Work

Use the same valid values in a preparation protocol, a teaching example and a downloadable tube figure.

serial dilution diagram: tenfold series

Plan a plate standard curve

Calculate a dilution series, then carry the concentrations into an editable 96-well plate layout for standards, blanks and replicates.

Explore this example
serial dilution diagram: twofold series

Prepare a lab setup figure

Keep the calculated tube series alongside a labeled lab setup diagram when preparing a protocol or methods figure.

Explore this example
serial dilution diagram: remaining volumes

Check remaining sample volume

Compare the freshly mixed volume with the remaining-volume column. Intermediate tubes have already supplied an aliquot to the next tube; the last tube has not.

Explore this example
serial dilution diagram: six step series

Create a protocol illustration

Export the tube diagram beside the preparation table. Labels show concentrations and onward transfer amounts from the same calculation.

Explore this example
Serial dilution series comparing 1000 and 2000 microliter prepared volumes

Teach cumulative dilution

Compare the factor used at one step with the cumulative factors across the series. The values show why repeated tenfold dilutions produce factors of 10, 100 and 1000.

Explore this example
serial dilution diagram: tenfold series

Keep a reproducible calculation

Save input values and units as JSON and export the full table as CSV. Reopen the plan when adapting the number of steps or prepared volume.

Explore this example

Worked Examples Prepared with SciFig

The serial dilution calculator example begins at 1000 µg/mL with four tenfold steps and 1000 µL prepared per tube. The concentrations are 100, 10, 1 and 0.1 µg/mL. I kept the table beside the tube figure so those values and the transfer labels describe the same calculation.

SciFig · Tenfold series

Figure preparation · SciFig

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Free Serial Dilution Calculator — Volumes & Diagram

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