Volcano Plot Generator Free

A volcano plot relates effect size to statistical significance for many tested features. In differential expression, each point often represents a gene or protein. Horizontal position commonly shows log2 fold change; vertical position commonly shows negative log10 of the chosen p-value. State whether that value is adjusted or unadjusted and identify the comparison direction.

Yes. Start free, no card required: SciFig DataChart (data-driven charts) plots your volcano plot straight from the effect sizes and p-values in your table. Map the columns, set thresholds and labels, then convert the result to SVG for full vector editing.

It plots your CSV. Give SciFig DataChart the effect sizes and p-values from your differential-expression analysis in a CSV, TSV, TXT or Excel table, map effect and p-value columns, identify adjusted or unadjusted values, and select the comparison direction and thresholds.

Name both the transformation and the comparison. For example, log2 fold change needs a stated numerator and reference group, while the vertical axis should identify negative log10 of adjusted or unadjusted p-values. A positive log2 fold change means higher values in the numerator group. A volcano plot online tool cannot infer these choices from the shape of the point cloud.

Use thresholds justified by your analysis plan and statistical method. There is no universal pair suitable for every study. If an educational figure shows threshold lines, label them as illustrative and make the point colors consistent with those lines. Do not let a volcano plot maker choose or invent thresholds for your research.

Yes. Refine the column mappings, thresholds, labels and style in SciFig DataChart, then regenerate from your table. Download PNG, PDF, TIFF or EPS and Python code, or convert the result to SVG for editing in SciFig Vector Canvas.