Western Blot Diagram Generator

A western blot diagram shows how one protein is detected in a mixture. It traces the sample through gel separation, transfer to a membrane, antibody binding, and band visualisation, so a reader can follow the whole workflow without reading the protocol text. A diagram of western blotting typically labels each stage in order.

Prepare, separate, transfer, block, probe, and detect. Lysate is denatured and loaded onto an SDS-PAGE gel that separates proteins by size, the separated proteins are transferred to a membrane, blocking prevents nonspecific binding, primary and secondary antibodies bind the target, and detection reveals bands. A western blotting flow chart puts these six stages in sequence.

An electric field drives proteins out of the gel onto the membrane. The gel and a nitrocellulose or PVDF membrane are sandwiched between filter paper and electrodes, and the current moves the separated proteins sideways so their positions are preserved. A western blotting technique diagram usually draws this sandwich in cross-section.

Describe the stages and labels you want in the figure. SciFig's western blot diagram generator lays out the workflow with the gel, membrane, antibodies, and detected bands drawn correctly, at publication resolution, so it can go straight into a methods section or a supplementary protocol.

The primary binds the target; the secondary carries the signal. A primary antibody recognises a specific epitope on the protein of interest, and an enzyme- or fluorophore-conjugated secondary antibody binds the primary to amplify and report it. SciFig's western blot diagram generator can show both layers stacked on the membrane-bound protein.

A western blot separates proteins by size first; an ELISA does not. Because the blot runs a gel before probing, it reports both the identity and the apparent molecular weight of the target, while an ELISA gives a quantitative signal from an intact well. A western blotting technique diagram makes that size-separation step explicit.