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PNAS Formatting Requirements 2026: Figures, DPI, Fonts

PNAS figure requirements: 8.7, 11.4, or 17.8 cm widths, 300–1200 ppi by image type, 6–12 point text, RGB color, and TIFF, EPS, PDF, or PPT files.

Alex Carter
Alex Carter
Founder and independent developer
PNAS figures use 300 ppi to 1200 ppi by image type.

The workflow is as important as the numbers: PNAS has manuscript page and display-element expectations, and the submission system handles figure source files separately for LaTeX. Prepare the art at the size you want published, then check the final file and the compiled manuscript together.

PNAS figure specifications at a glance

A scientific figure for PNAS must remain legible after final-size reduction.

ParameterRequirement
One-column width8.7 cm
One-and-a-half-column width11.4 cm
Two-column width17.8 cm
Recommended maximum height22.5 cm
Text after reduction6 points to 12 points
Halftone without type300 ppi
Combination halftone600–900 ppi
Line art1000–1200 ppi
Color modeRGB
Accepted figure filesTIFF, EPS, PDF, or PPT
Minimum vector line weight0.25 points

PNAS's digital guide prefers vector files because they remain scalable. It also asks authors to embed fonts in vector files and keep the same font across the manuscript's figures.

How wide can a figure be? Single vs double column

Use 8.7 cm for one column, 11.4 cm for one-and-a-half columns, or 17.8 cm for two columns. Keep the height below the recommended 22.5 cm maximum so the legend and page layout remain workable.

Prepare the artwork at its intended final size rather than using the full page as a canvas. PNAS can decide the final published size and may ask authors to shorten a manuscript that exceeds the length requirement.

What's the minimum font size inside a figure?

After reduction, numbers, letters, and symbols should be no smaller than 6 points and no larger than 12 points. The Digital Art guide separately recommends preparing text at 6–8 points or larger so it remains legible after reduction. Keep type size consistent within a graphic. The guide lists standard fonts such as Arial, Helvetica, Times, Symbol, Mathematical Pi, and European Pi, and asks authors to embed fonts in vector files.

The lower bound protects legibility; the upper bound keeps the graphic from carrying oversized display text. Check the reduced figure, not only the source canvas.

Which file formats and resolutions does PNAS accept?

The submission guidance accepts TIFF, EPS, PDF, or PPT for figures and schemes. The digital guide prefers vector images such as EPS, AI, and PDF for line art and editable text.
Use 300 ppi for grayscale or color images without type, 600–900 ppi for grayscale or color images with type, and 1000–1200 ppi for line art such as bar graphs. Use RGB for color image files and LZW compression rather than JPEG compression when saving the preferred raster formats.

Does PNAS require a graphical abstract?

The PNAS submission page lists the manuscript components, figures, tables, and legends, but it does not list a separate graphical abstract as a required component. Do not confuse an article figure with an optional cover image: PNAS handles cover images through a separate invitation and submission path.

If your result needs a visual summary, use an ordinary figure with a clear legend and call it out in the manuscript. Do not add an unlabeled graphical abstract file to the submission package unless the current submission system explicitly requests it.

How many figures can you include?

PNAS does not give one universal figure count for every article type on the public page. A standard six-page Research Report is described as approximately 4 medium-size graphical elements, while the journal allows up to 12 pages for a Research Report. Treat that example as an article-type planning anchor, not as a promise that every paper has the same cap.
Brief Reports are limited to 3 pages, approximately 1,600 words, and 15 references; their supporting information is limited to extended methods, essential datasets, and videos, with no additional tables or figures.

Supplementary, appendix, and extended data figures

PNAS calls its supplementary package an SI Appendix. Refer to it in the manuscript, number supporting figures and tables as S1, S2, and so on, and keep the main article self-contained without relying on the SI for its central logic.

For LaTeX submissions, upload each figure source separately as the Figure file type and keep tables in the main manuscript file. The submission page also requires data-sharing plans for data, documentation, and code used in the analysis.

Word and page limits by article type

Article typeTypical or maximum lengthOther limits stated by PNAS
Research Reportpreferred 6 pages, maximum 12 pagesabout 4,000 words, 50 references, 4 medium-size graphical elements for a standard 6-page example
Brief Report3 pagesabout 1,600 words, 15 references, no additional SI tables or figures
Letter to the Editor—500 words, 2 graphical elements, 10 references

These are article-type rules, not a single journal-wide word limit. Read the relevant article-type description before choosing the layout.

Templates: LaTeX or Word?

PNAS provides a LaTeX template and an Overleaf workflow. The submission system runs PDFLaTeX, so the author page advises using the latest LaTeX2e and the supplied class, style, bibliography, and support files. For Word or RTF, keep tables editable and upload figure files in the accepted figure type.

Use the official file that matches your article type:

Avoid nesting, cross-referencing, and input commands that can cause LaTeX compilation errors. If the system-generated PDF is incomplete, PNAS allows an alternate PDF as a related manuscript file.

Title page, cover letter, and highlights

The initial package should include a title page, abstract, significance statement for Direct and Contributed Submissions, main text, references, figure legends, contact and competing-interest information, data-sharing plans, funding and license information, and at least three appropriate Editorial Board members, NAS members, and qualified reviewers.

The title should be under 135 characters, the abstract no more than 250 words, and the significance statement no more than 120 words when required. The public submission checklist does not list a separate highlights file.

Formatting quirks specific to PNAS

PNAS has three production quirks worth checking before upload:

  • Figure source files are handled separately for LaTeX, while tables remain in the main manuscript file.
  • The publication-ready guide expects images at final size and may reduce figures to fit the article length.
  • PNAS includes alternative text for journal figures in the version of record beginning with Volume 123; production generates and reviews that text.

Keep source files, fonts, and the compiled PDF together until the production team confirms the final version.

Figure problems that trigger a revision request

The digital guide identifies low-resolution images as a leading cause of art resubmission and schedule delays. Typical failures include a raster image below the right ppi band, unembedded fonts, RGB conversion mistakes, a line below 0.25 points, an oversized canvas, and a figure whose text becomes unreadable after reduction.

PNAS also does not recommend figures submitted in Microsoft Office because they may take additional processing time and can delay publication. Use vector artwork for line art whenever possible.

Build a PNAS-ready figure

  1. Set the final canvas to 8.7 cm, 11.4 cm, or 17.8 cm wide and keep its height below 22.5 cm.
  2. Use RGB and choose 300 ppi, 600–900 ppi, or 1000–1200 ppi according to the image type.
  3. Keep text between 6 points and 12 points after reduction, embed the fonts, and keep vector lines at least 0.25 points.
  4. Export one of TIFF, EPS, PDF, or PPT, with vector artwork preferred for text and line art.
  5. Check the source figure, the compiled PDF, the legend, and the article-type page limit as one package.

Keep the source file editable

Final-size vector artwork makes PNAS width, type, line, and font checks possible before production.

Try Free
Prepare numerical figures with SciFig DataChart (data-driven charts): AI plans the chart and Python plots every value precisely from your original table, and keep experimental images tied to their original acquisition files. For projects whose destination permits AI-generated figures, SciFig AI Illustration (AI scientific illustration) creates conceptual artwork you can convert to editable SVG; measured imagery stays in the original scientific image-processing workflow. See PNAS submission guide, PNAS Impact Factor guide.
For figure preparation, SciFig offers SciFig DataChart for scatter plots, box plots, and heatmaps generated precisely from your table data, SciFig FlowChart (flowcharts and diagrams) for study workflows and diagrams as native, editable SVG, and SciFig AI Illustration for mechanism and conceptual artwork you can convert to editable SVG. SciFig DataChart uses AI to plan the chart and prepare plotting code, then Python draws it from your original table. SciFig FlowChart uses AI to generate the SVG, while SciFig AI Illustration generates images with AI. SciFig DataChart supports 49 scientific chart types and exports PNG, PDF, EPS, and TIFF, and it gives you the table, plotting settings, and Python code, so your figure source files stay complete.

Does PNAS allow AI-generated figures?

PNAS does not permit AI-generated images or graphics in a standard submission. Its policy says AI tools for creating images or graphics may not be used unless the software is the subject of the work under consideration. PNAS also does not permit AI-generated content in cover art submissions. PNAS requires authors who used AI or generative AI during the research process to disclose the tool name and model or version in Materials and Methods or Acknowledgments.

If your figure already exists:

  • Raster figure that needs an editable SVG: use Vector Canvas for AI vectorization into an editable vector figure, with labels, elements, and layers ready for further editing. An existing SVG can be edited and exported manually in the same canvas.
  • Existing figure that needs a quality boost: Figure Enhancer uses AI to generate the enhanced image, with cleanup, upscaling, and export up to 8K.
  • Numerical charts from your own data: SciFig DataChart (data-driven charts) uses AI to plan the chart and prepare plotting code, then Python draws it precisely from your original table, so every plotted value is your data. Because AI takes part in the planning step, PNAS's image and disclosure policy above is the reference for whether and how to use it in a submission.
  • Other uses: These editing workflows also support grant figures, posters, defense slides, theses, and lab pages. For those, SciFig Text-to-Figure creates a new figure you can edit element by element, and each venue's AI policy is a useful reference.

Frequently asked questions

Sources

This page uses official PNAS pages captured through a rendered browser session and reviewed in 2026.

See our editorial standards for how these guides are researched and corrected.

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