Flow Cytometry Plot

A flow cytometry plot is a graph that shows individual cells as dots positioned by two measured signals, such as two fluorescence markers. Dense clouds of dots reveal distinct cell populations within a sample.

A FACS plot is a flow cytometry scatter plot from fluorescence-activated cell sorting. It usually plots two fluorescent markers against each other and uses gates — often quadrants — to define and quantify cell populations.

Read a flow cytometry plot by looking at where clusters of dots fall relative to the axes and gates. Each quadrant or gate represents a population, and the percentage of events in each gate quantifies that population.

Quadrant gating divides a two-parameter flow cytometry plot into four regions — double-negative, single-positive for each marker, and double-positive — so you can count the fraction of cells in each population.

Yes. The icon is fully editable in SciFig — recolor the populations, relabel the axes and gates, resize it, and export it as SVG or transparent PNG to drop into any figure, poster, or slide.

You can export the flow cytometry plot icon as an editable SVG for clean scaling in vector layouts, or as a transparent PNG to place over any background in slides and posters.